Mitochondria acquire the vast majority of their proteins from the cell cytosol in a manner that requires the coordinated function of numerous translocation machineries embedded within their two membrane systems. One of the most multifaceted of these machineries is the Translocase of the Inner Membrane 23 TIM23 complex, which mediates the import of both inner membrane- and matrix-targeted proteins. The central subunit of this complex is Tim23, a voltage-gated channel-forming protein that constitutes part of the protein-conducting channel residing in the inner membrane. Tim23 has bipartite domain organization with a C-terminal integral membrane domain and an N-terminal intrinsically disordered domain that resides in the intermembrane space, termed Tim23 IMS.
Master of Science in Biochemistry and Molecular Biology (MSc)
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Neurotransmitters are initially stored within each synaptic vesicle and their release at synapse is mediated by membrane fusion between synaptic vesicle membrane and plasma membrane. If solely depending on thermal fluctuation, the fusion process would take days on average. This is contrary to the physiological sub-millisecond timescale, which is much faster than any other biological membrane fusion process. All together, they form a delicate synaptic fusion machinery. However, how these proteins cooperate to mediate fast synaptic vesicle fusion is still not fully understood. The aim of my thesis research is to understand how these proteins collaborate to initiate SNARE protein assembly, one key aspect of this fusion process.
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Fu, Ziao. Single-particle reconstruction technique is one of the major approaches to studying ribosome structure and membrane proteins. In this thesis, I report the use of time-resolved cryo-EM technique to study the structure of short-lived ribosome complexes and conventional cryo-EM technique to study the structure of ribosome complexes and membrane proteins. The thesis consists three parts.
A membrane protein is a protein molecule that is attached to or associated with the membrane of a cell or an organelle. They represent about a third of the proteins in living organisms. Examples of membrane proteins include ion channels, receptor proteins, and proteins that allow cells to connect to each other.